Browsing by Author "Adedayo MR"

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    Antibacterial potency and phytochemical screening of the bark of Terminalia catappa against some clinical isolates
    (Advanced Research Journals, 2016) Ajiboye AE; Babatunde SK; Adedayo MR; Adetumbi MA; Ajuwon IB; Ajasegun T
    Context and purpose of the study: To evaluate the antibacterial properties of ethyl acetate and aqueous extracts of the bark of Terminalia catappa against some clinical isolates. Main findings: The antibacterial activity of the T. catappa bark extracts was five bacterial clinical isolates which are Escherichia coli, Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa and Klebsiella pneumoniae using agar-well diffusion method. The minimum inhibitory concentration was done by the broth dilution method. Broad spectrum antibiotics were used as positive control for the antibiotic sensitivity test. Qualitative and quantitative phytochemical screening of T. catappa bark both were carried out using standard methods. However, ethyl-acetate and aqueous extracts of Terminalia catappa showed that K. pneumoniae was more sensitive compared with E. coli, S. typhi, S.aureus and P. aeruginosa. Antibacterial activity of the ethyl acetate and aqueous extracts showed the value range from 10.33 ± 0.58 mg/ml to 9.33 ± 0.60 mg/ml. Ethyl acetate extract showed minimum inhibitory concentration (MIC) at 80 mg/ml while the aqueous extract shows MIC at the concentration of 100 mg/ml. However, the minimum bactericidal concentration (MBC) of ethyl-acetate extract against the clinical isolates was at the concentration of 100 mg/ml while there was no minimum bactericidal concentration (MBC) for the aqueous extract. Phytochemical screening shows the presence of saponins, glycosides, and alkaloids, in larger quantity while flavonoids, tannins, and steroids in smaller quantity. Brief summary and potential implications: This study indicates that the extracts were efficacious and can be used for the management of diseases caused by the tested organisms. Results obtained support the use of this plant as use in traditional medicine and support that the plant extracts possess compounds with good antimicrobial properties that can be used as antimicrobial agents in the search for new antimicrobial drugs.
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    Antibacterial screening of Phoenix dactylifera L. (Date palm) seed extracts on some bacterial isolates associated with dental caries
    (Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., 2020) Adedayo MR; Ajiboye AE; Adetula FE
    Dental diseases refer to conditions of deterioration and disintegration of the oral cavity due to the activities of pathogenic microbes. This study was carried out to determine the antibacterial activity of aqueous and ethanol extracts of Phoenix dactylifera L. (Date palm) seed on selected bacteria isolates associated with dental caries namely Staphylococcus aureus, Streptococcus mutans and Lactobacillus casei. Bioactive components of the seed were extracted using water and ethanol as solvents. The antibacterial activity of the extracts was examined by agar well diffusion method. The minimum inhibitory and bactericidal concentrations (MIC and MBC) were also determined using standard methods. All extracts were active against the tested isolates at the concentrations examined. L. casei was the most susceptible organism followed by S. mutans. S. aureus was the least susceptible. Ethanolic extract was more effective than aqueous extract at all the concentrations tested in the study. The MIC of aqueous and ethanolic extracts was 20 mg/ml while the MBC was 80 mg/ml respectively against all the bacterial isolates tested. It can be concluded that the aqueous and ethanolic extracts of the seed possess antibacterial potential against all the test organisms and could be useful in treatment of dental diseases and other related diseases caused by the bacteria isolates tested in the study.