Browsing by Author "Aborisade, W. T."

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    EFFECTS OF AN ORGANOCHLORINE AND PYRETHROID PESTICIDE FORMULATION ON SOIL’S CULTURABLE MICROBIAL POPULATION
    (International Journal of Technical Research & Science, 2020) Aborisade, W. T.; Atuanya, E. I.
    Non-target effect of pesticide on soil organisms is an important area of ecotoxicology, due to their roles in ecosystem sustainability. Thus, the effects of an organochlorine (endosulfan) and a synthetic pyrethroid (cypermethrin) pesticide formulation on soils’ culturable microbial population were investigated. The study was carried out on loamy sand soil sample types collected from Ondo State, Forest Reserve, Owena, Nigeria. Pesticide application rates in the range of half of the normal field rate (0.5FR), the normal field rate (FR), two times the normal field rate (2FR), four times the normal field rate (4FR) and eight times the normal field rate (8FR) were applied to the soil microcosm design in the laboratory; while the untreated soil serve as control. The experiment set up was completely randomized in three replicates and soil samples were collected from each of the set up at 5 days interval over 35 days period of exposure for analysis. The pour plate technique was used for the enumeration of bacterial and fungal colonies on nutrient agar and potato dextrose agar respectively. The results indicated that both pesticides had effects on the population of bacterial and fungal counts of the soil samples with significant (p < 0.05) adverse effect observed at the treatment rate above the normal field application rate. At lower application rates (0.5FR, FR and 2FR), temporary inhibitory effect on bacterial and fungal population were observed. The progressive increase in inhibitory effect with corresponding increase in concentration of endosulfan and cypermethrin were noticed.
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    Effects of an organophosphate (glyphosate) and a quaternary ammonium (paraquat) herbicides formulation on soils’ culturable bacterial and fungal populations
    (International Research Journal of Biological Sciences, 2022) Aborisade, W. T.; Atuanya, E. I.
    Herbicides play significant roles in weed management and contribute immensely to increase in productivity in agronomy system. However, its continuous application could have some dire effect on non-target soil microbiota. Therefore, this study assessed the impact of glyphosate and paraquat herbicides on soil culturable bacterial and fungal population. The loamy composite soil samples of forest reserve, Owena, Ondo State, Nigeria were used for the experiment. Each of the herbicide formulation was applied at the concentration rates ranged from half of the field recommended rate (0.5FR), the recommended rate (FR), two, four, and eight times the recommended rate (2, 4 and 8) FR respectively. The treatments were replicated thrice and arranged in complete randomized design, while the untreated soil samples serve as control. The standard pour plate technique was used for the enumeration of bacterial and fungal colonies after 5, 10, 15, 20, 25, 30 and 35 days of exposure. The findings showed that the glyphosate pesticides formulation applied at concentration range of 0.5 to 2FR significant stimulate bacterial populations of the soil samples while the fungal populations was not affected at the same concentration. However, the treatments at higher doses (4FR and 8FR) significantly reduced the number of bacterial and fungal counts of the soil samples. For paraquat treated soils, the treatment rate below the double recommended field rate did not have any significant effect (p > 0.05) on both bacterial and fungal populations. While the increases in inhibitory effect were observed with corresponding increases in paraquat application rates in the soil samples.
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    Isolation and Screening for Potential endosulfan-degrading bacteria from Soil
    (Dutse Journal of Pure and Applied Sciences, 2024) Aborisade, W. T.; Ajao, A. T.; Ntagbu, F. G.; Bale, S. I
    The urgent need for sustainable management of persistent organic pollutants, due to their negative impact on the environment, is of great concern to environmental stakeholders. In this study, the potential endosulfan-degrading (PED) bacteria were screened and identified from fresh soil samples collected from the Teaching and Research farm of Kwara State University, Nigeria. The bacterial strains were isolated using enrichment techniques and characterized through morphological, biochemical, and molecular analyses. Their potential for endosulfan utilization was assessed in an endosulfan supplemented mineral salt medium. Three potential endosulfan-degrading (PED) bacterial strains isolated were Achromobacter xylosoxidans strain PED1, Pseudomonas alkylphenolica strain PED2, and Sphingomonas sp. Strain PED3 with the accession numbers MF973060, MF973061 and MF973062 respectively. The optical density (OD) values increased for all the isolates by the third day, and the highest OD values of 0.51 (PED1), 0.52 (PED2) and 0.59 (PED3) were recorded on the ninth day. The observed increase in population density of the isolates in the endosulfan-supplemented mineral salt medium suggests their capability to utilize the endosulfan compound. Further study is required to evaluate the suitability of these strains for bioremediation purposes.
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    Water quality assessment and bacteriological evaluation of fishpond in Ilorin
    (Aswan University Journal of Environmental Studies (AUJES), 2023) Idemudia, I. B.; Aborisade, W. T.; Ajao, A. T.
    Good water quality is essential for a productive aquaculture system and to safeguard the health of the consumer. We assessed the quality of fishpond water in Phase 1, Mubo-Royal Valley fish farm in Ilorin, Kwara State, Nigeria. Water samples were collected in the 1st and 3rd weeks of fish breeding from three earthen and two concrete fishponds. The bacteriological and physicochemical parameters of the samples were determined in line with standard methods. Results showed that the pH, temperature, and dissolved oxygen (DO) values were within the recommended range of the National Environmental Standard and Regulations Enforcement Agency (NESREA). However, higher chemical oxygen demand (COD) values above the NESREA recommended range were recorded in the 1st and 3rd weeks of sampling in earthen Pond 1 and Pond 2, respectively. The Nitrate (NO3 2- ) and phosphate (PO4 2- ) contents of all sampling ponds were within the acceptable values of NESREA in the 1st week of sampling. While significant (p < 0.05) increases in NO3 2- and PO4 2- were observed in earthen ponds in the 3rd week. Copper and iron (mg/l) values were higher than NESREA recommended range, while lead and chromium values were within the range. Bacterial loads varied with the period of sampling with significant (p < 0.05) increases in the population of heterotrophic bacteria and Salmonella Shigella in the 3rd week of sampling. The nine bacterial genera isolated were Pseudomonas sp., Micrococcus sp., Staphylococcus sp., Salmonella sp., Escherichia coli, Enterobacter sp., Proteus sp., Streptococcus sp. and Bacillus sp. All the isolates were present in earthen and concrete ponds except Proteus sp. and Streptococcus sp. were absent in concrete ponds. Pseudomonas sp. had the highest frequency of occurrence while Streptococcus sp. had the lowest. Regular monitoring of water quality parameters is recommended to prevent potential risks associated with potential pathogens in fishponds