Browsing by Author "A.T. Ajao, W.T. Aborisade, N.A.Moshood"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    ENHANCING BIODEGRADATION EFFICIENCY OF REACTIVE BLACK-5 DYE USING BACILLUS WIEDMANNII STRAIN NAF4: OPTIMIZATION OF DEGRADATION PARAMETERS VIA RESPONSE SURFACE METHODOLOGY (RSM)
    (2024) A.T. Ajao, W.T. Aborisade, N.A.Moshood
    Reactive Black 5 (RB-5) dye is widely used in industries such as textile, paper, and leather, raising environmental concerns due to its persistence and adverse effects. This study aimed to develop efficient and eco-friendly strategies for RB-5 dye removal from industrial wastewater. RB-5 dye-degrading bacteria, namely NAF1, NAF2, NAF3, and NAF4, were isolated from soil contaminated with textile effluents. Evaluation of their decolorization potential revealed NAF4 as the most effective, achieving a decolorization percentage of 89%, followed by NAF3 and the co-culture at 75% and 73%, respectively. The isolate NAF4 showed significant production of tyrosinase and laccase enzymes. The 16SrRNA sequencing confirmed the identities of the isolates belonging to the following genera Bacillus, Pseudomonas, Escherichia, and Citrobacter. The biodegradation potential of B. wiedmannii strain NAF4 for RB-5 dye was assessed using Response Surface Methodology (RSM). The optimized conditions for RB-5 degradation were determined to be an agitation speed of 115.777 rpm, pH of 7.449, inoculum size of 12.255, and temperature of 29.74°C. The RSM model exhibited high statistical significance with an F-value of 53.30 and low p-values (<0.0001) as well as a correlation coefficient (R²) value of 0.9813. Validation studies confirmed the adequacy and precision of the model. The maximum RB-5 degradation achieved was 90.2291%. This study provides insights into the potential applicability of RSM for optimizing degradation processes in various contexts and offers promising solutions for RB-5 dye removal from industrial wastewater, mitigating its environmental impact.