Phytochemicals and anticancer activity of methanol extract of Trigonella foenum-greacum seed on breast cancer cell lines

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dc.contributor.authorMutiu A. Alabi, Marvis A. Arowolo, Asiat Na’Allah, Pranav Kumar Prabhakar, Eberechukwuka G. Linus, Naga Raju Maddela, Sesan A. Aransiola, Hassan T. Abdulameed, Beloved K. Ajani and Ram Prasad
dc.date.accessioned2023-07-19T17:03:04Z
dc.date.available2023-07-19T17:03:04Z
dc.date.issued2023-07-13
dc.description.abstractThis study was aimed to examine the anticancer potential of Trigonella foenum-greacum seed methanol extract by analyzing its phytochemicals and its breast cancer cells effects. The anti-cancer efficacy of the extract was tested on fibroblast cells and MCF-7 through various cellular assays, including apoptosis, mitochondrial membrane potential, reactive oxygen species, cell cycle distribution, and cell cytotoxicity assay were all investigated. By using GC-MS analysis, fifty-five bioactive compounds were identified in the extract. When the cancer cell lines were treated with the extract, the typical apoptotic morphology was observed, this resulted in a dose-dependent reduction of cancer cell migration and adhesion. Flow cytometry studies revealed that the extract significantly induced apoptosis by triggering a change from G2/M to polyploidy (>G) at greater doses. Furthermore, the extract increased mitochondrial depolarization and ROS levels. This study demonstrates the extract’s anti-cancer properties and highlights the need for further analysis to understand how Trigonella foenum-greacum seed extract induces program cell death through multiple signaling pathways at varying concentrations.
dc.identifier.citationAlabi, M.A., Arowolo, M.A., Na’Allah, A., Prabhkar, P.K., Linus, E.G., Aransiola, S.A., Abdulameed, H.T., Ajani, B.K., Maddela, N.R. and Prasad, R. (2023). Phytochemicals and Anticancer Activity of Methanol Extract of Trigonella foenum-greacum Seed on Breast Cancer Cell Lines, South African Journal of Botany. 160; 272-287. Published by Elsevier B.V. Available online: https://www.sciencedirect.com/science/article/abs/pii/S025462992300399X?via%3Dihub. Doi: 10.1016/j.sajb.2023.07.021. Scimago Journal Rank - Q2.
dc.identifier.urihttps://kwasuspace.kwasu.edu.ng/handle/123456789/617
dc.language.isoen
dc.publisherSouth African Journal of Botany
dc.titlePhytochemicals and anticancer activity of methanol extract of Trigonella foenum-greacum seed on breast cancer cell lines
dc.typeArticle
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