Ovarian Metabolic activity in Dehydroepiandrosterone-Induced Polycystic Ovary in Wistar rats Treated with Aspirin
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Date
2019
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JBRA Assisted Reproduction
Abstract
Objectives: Polycystic ovary syndrome (PCOS) rep
resents 75% of the cases of anovulatory infertility. The aim
of this study was to investigate the role of aspirin on de
hydroepiandrosterone (DHEA) - induced polycystic ovary
syndrome in Wistar rats.
Methods: Twenty eight (28) pre-pubertal female Wis
tar rats of 21 days old weighing 16 - 21 g were divided into
4 groups (7 rats/group) and treated as follows; group I
received distilled water and served as Control; Group II re
ceived 6 mg/100 g body weight DHEA in 0.2 ml of oil sub
cutaneously to induce PCOS. Group III received 7.5 mg/
kg of aspirin orally; Group IV received 6 mg/100kg of body
weight of DHEA in 0.2ml of oil subcutaneously and 7.5 mg/
kg of aspirin orally. After 15 days of administration, the
rats were slaughtered by cervical dislocation. Blood sam
ples and ovaries were collected for reproductive hormonal
analysis, biochemical and histopathological analysis. The
expressions of mRNA androgen receptor (AR) gene in the
ovary were determined by real time reverse transcriptase
polymerase chain reaction (qPCR). All the data was an
alyzed using one way ANOVA with the Graph pad prism
software version 6. A p<0.05 was considered significant.
Results: The results obtained showed that dehydroe
piandrosterone treatment caused significant decrease
(p<0.05) in total protein, superoxide Dismutase (SOD),
glutathione-s- transferase (GST), Ca2+ ATPase, and signif
icant increase (p<0.05) in malondialdehyde, vascular en
dothelial growth factor, tumor necrosis factor and estrogen
as compared to Controls. The group co-administered with
DHEA and aspirin showed significant increases in SOD,
GST, CAT, GSH, Progesterone, Ca2+ ATPase, Na+ ATPase,
H+ ATPase and significant reduction (p<0.05) in malondi
aldehyde, VEGF, TNF-α and estrogen as compared with the
DHEA group. The histopathological analysis showed reduc
tions in cystic fibrosis, atretic ovaries, increased expression
of Bcl-2 and E- Cadherin and reduced Bax expression in the
group that received Aspirin and DHEA.
Conclusion: This study clearly demonstrates that
Aspirin has ameliorating effects against polycystic ovary
syndrome via anti-inflammatory and hormonal modulatory
pathways.
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